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Literature summary for 3.5.1.24 extracted from
- Characterization of bile salt hydrolase from Lactobacillus gasseri FR4 and demonstration of its substrate specificity and inhibitory mechanism using molecular docking analysis (2017), Front. Microbiol., 8, 1004 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetone | slight activation | Lactobacillus gasseri |  |
| butanol | - |
Lactobacillus gasseri | |
| DTT | slight activation at 10 mM | Lactobacillus gasseri | |
| Isopropanol | - |
Lactobacillus gasseri | |
| Triton X-100 | - |
Lactobacillus gasseri | |
| Tween 80 | - |
Lactobacillus gasseri | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene bsh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Eschericchia coli strain DH5alpha | Lactobacillus gasseri |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ampicillin | almost complete inhibition | Lactobacillus gasseri | |
| ascorbic acid | moderate inhibition | Lactobacillus gasseri | |
| CaCl2 | slight inhibition | Lactobacillus gasseri | |
| CuCl2 | strong inhibition | Lactobacillus gasseri | |
| CuSO4 | strong inhibition | Lactobacillus gasseri | |
| doxycycline hydrochloride | almost complete inhibition | Lactobacillus gasseri | |
| erythromycin | moderate inhibition | Lactobacillus gasseri | |
| ethanol | slight inhibition | Lactobacillus gasseri | |
| KIO3 | strong inhibition | Lactobacillus gasseri | |
| Lincomycin | slight inhibition | Lactobacillus gasseri | |
| methanol | slight inhibition | Lactobacillus gasseri | |
| MgCl2 | - |
Lactobacillus gasseri | |
| MgSO4 | - |
Lactobacillus gasseri | |
| MnCl2 | - |
Lactobacillus gasseri | |
| MnSO4 | - |
Lactobacillus gasseri | |
| additional information | chloroform, 2-mercaptoethanol and EDTA show poor effects on the enzyme activity. Proteinase K almost completely abolishes the enzyme activity, strong inhibition by pepsin. The enzyme is not affected by alpha-amylase and catalase, and only slightly by lysozyme | Lactobacillus gasseri | |
| NaHIO3 | strong inhibition | Lactobacillus gasseri | |
| neomycin | strong inhibition | Lactobacillus gasseri | |
| oxytetracycline | almost complete inhibition | Lactobacillus gasseri | |
| Penicillin V | almost complete inhibition | Lactobacillus gasseri | |
| riboflavin | almost complete inhibition | Lactobacillus gasseri | |
| SDS | almost complete inhibition | Lactobacillus gasseri | |
| ZnCl2 | - |
Lactobacillus gasseri | |
| ZnSO4 | - |
Lactobacillus gasseri | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycocholate + H2O | Lactobacillus gasseri | - |
cholate + glycine | - |
? | |
| glycocholate + H2O | Lactobacillus gasseri FR4 | - |
cholate + glycine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactobacillus gasseri | B9V405 | - |
- |
| Lactobacillus gasseri FR4 | B9V405 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Lactobacillus gasseri |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycocholate + H2O | - |
Lactobacillus gasseri | cholate + glycine | - |
? | |
| glycocholate + H2O | - |
Lactobacillus gasseri FR4 | cholate + glycine | - |
? | |
| additional information | purified recombinant LgBSH exhibits substrate specificity toward glyco-conjugated bile salts (GCA and GDCA) compared to tauro-conjugated bile salts. Substrate specificity of LgBSH, overview | Lactobacillus gasseri | ? | - |
? | |
| additional information | purified recombinant LgBSH exhibits substrate specificity toward glyco-conjugated bile salts (GCA and GDCA) compared to tauro-conjugated bile salts. Substrate specificity of LgBSH, overview | Lactobacillus gasseri FR4 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37000, recombinant enzyme, SDS-PAGE | Lactobacillus gasseri |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bile salt hydrolase | - |
Lactobacillus gasseri |
| BSH | - |
Lactobacillus gasseri |
| LgBSH | - |
Lactobacillus gasseri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 52 | - |
recombinant enzyme | Lactobacillus gasseri |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | activity range, profile overview | Lactobacillus gasseri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
recombinant enzyme | Lactobacillus gasseri |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | activity range, profile overview | Lactobacillus gasseri |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | enzyme structure homology model of LgBSH using the structure of Enterococcus faecalis BSH (PDB ID 4WL3) as a template. Residues involved in catalysis are identified based on the superimposed structure, including Cys1, Arg16, Asp19, Asn79, Asn171, and Arg224. Molecular docking analysis of ligands | Lactobacillus gasseri |
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